![]() ![]() Cartilage contact stress in retroverted hips for all subjects and loading scenarios. The fringe plots shown in the walking mid, descending stairsand chair rise rows were averaged to create Figure 5. ![]() In conclusion, CAXPCi holds a strong potential for being adopted as a routine laboratory tool for non-destructive, high throughput assessment of 3D structural changes in murine articular cartilage, with a possible impact in the field similar to the revolution that conventional microCT brought into bone research. Cartilage contact patterns were subject-specific, but tended to be fairly distributed over the articular surface. From a technological standpoint, by showing the capability of the system to detect cartilage also in water, we demonstrate phase sensitivity comparable to other lab-based phase methods (e.g. Following these successful proof-of-concept results in rat cartilage, we expect that an upgrade of the system to higher resolutions (currently underway) will enable extending the method to the imaging of mouse cartilage as well. Read more related scholarly scientific articles and abstracts. The loss of GAGs from these joints is the hallmark of. Histochemical visualization of the cartilage hyaladherins using a biotinylated hyaluronan oligosaccharide bioaffinity probe. Moreover, we show that small, surgically induced lesions are also correctly detected by the CAXPCi system, and we support this finding with histopathology examination. Articular or joint cartilage is the smooth hydrated tissue in the ends of bones in load-bearing joints, such as knees, hips and shoulders. MRI allows detailed, multiplanar analysis of the joint anatomy, as well as cartilage and underlying bone, with the ability to view articular surfaces at any angle. We show that a simple laboratory system based on coded-aperture x-ray phase contrast imaging (CAXPCi) can correctly visualize the cartilage layer in slices of an excised rat tibia imaged both in air and in saline solution. Magnetic Resonance Imaging (MRI) is the most widely used non-invasive tool to assess intra articular soft tissues, and cartilage in particular. ![]() However, due to limitations in imaging technology, high-throughput 3D structural imaging has not been achievable in small rodent models, thereby limiting their translational potential and their efficiency as research tools. The total knee joint cartilage volume ranged from 16.6 to 31.4 ml, the size of the articular surfaces from 102 to 163 cm(2), and the mean cartilage thickness from 1.57 to 2.43 mm. These models are becoming essential tools for the development of new drugs for OA, a disease affecting up to 1/3 of the population older than 50 years for which there is no cure except prosthetic surgery. Being able to quantitatively assess articular cartilage in three-dimensions (3D) in small rodent animal models, with a simple laboratory set-up, would prove extremely important for the development of pre-clinical research focusing on cartilage pathologies such as osteoarthritis (OA). ![]()
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